Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Long Non-coding RNA HOXA11 Antisense Promotes Cell Proliferation and Invasion and Predicts Patient Prognosis in Serous Ovarian Cancer

doi: 10.4143/crt.2016.263

Figure Lengend Snippet: Knockdown of HOXA11 antisense ( HOXA11as ) inhibits serous ovarian cancer cell proliferation. (A) Expression of HOXA11as in human ovarian surface epithelial cell line (HOSE) and six ovarian cancer cell lines determined by quantitative real time polymerase chain reaction (qRT-PCR). (B) Knockdown efficiency was determined by qRT-PCR analysis in OVCA429 and SKOV3 cells. Cells were transfected with HOXA11as -specific siRNA (siHOXA11as) and negative control siRNA (siNC). (C, D) Knockdown of HOXA11as significantly reduced cell proliferation in OVCA429 and SKOV3 cells as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Bars indicate mean±standard deviation of three independent experiments performed in triplicate. * p < 0.05 vs. siNC. siHOXA11as, HOXA11as -specific siRNA.

Article Snippet: Normal human ovarian surface epithelial (HOSE) cells were purchased from ScienCell Research Laboratories (San Diego, CA).

Techniques: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, MTT Assay, Standard Deviation

Journal: The Journal of Molecular Diagnostics : JMD

Article Title: Improving Specificity for Ovarian Cancer Screening Using a Novel Extracellular Vesicle–Based Blood Test

doi: 10.1016/j.jmoldx.2024.09.001

Figure Lengend Snippet: Linearity of the OC Test. Plasma was spiked with extracellular vesicles (EVs) isolated from the COV413A ovarian cancer cell line to cover the full range of OC Test scores.

Article Snippet: Four different replicates of the highest concentration sample in the dilution series were made by spiking in EVs that had been previously isolated from conditioned media obtained from the culture of the COV413A human epithelial serous ovarian carcinoma cell line (used under license from the European Collection of Authenticated Cell Cultures) and quantitated on the Spectrodyne nCS1 particle counter (Signal Hill, CA), as previously described.

Techniques: Clinical Proteomics, Isolation

Journal: Radiation Oncology Journal

Article Title: PA1 cells containing a truncated DNA polymerase β protein are more sensitive to gamma radiation

doi: 10.3857/roj.2021.00689

Figure Lengend Snippet: Cell cycle analysis by flow-cytometry. (A) Analysis of the cell cycle of PA1 cells after 48 hours of γ-radiation treatment. Cells were arrested in the G2/M phase with increasing doses of radiation. (B) The graph showed significantly more cells were arrested in the G2/M phase in the case of 10 Gy and 15 Gy of radiations (n = 3; ** p < 0.001). (C) PA1PolβΔ cells after 48 hours of γ-radiation treatment. (D) In the case of the PA1PolβΔ cell cycle, the G2/M phase was arrested in significantly more cells in every dose of radiations (n = 3; ** p < 0.001).

Article Snippet: PA1 cells were obtained from the National Centre for Cell Science, Pune, India.

Techniques: Cell Cycle Assay, Flow Cytometry

Journal: Radiation Oncology Journal

Article Title: PA1 cells containing a truncated DNA polymerase β protein are more sensitive to gamma radiation

doi: 10.3857/roj.2021.00689

Figure Lengend Snippet: Apoptosis analysis by Annexin V-FITC/PI using flow-cytometry after 48 hours of treatment. All results are expressed as mean ± standard deviation three individual experiments, and error bar indicating standard deviation ( * p < 0.05, ** p < 0.001). (A) Control cells, i.e., non-treated cells, are mostly live cells. (B) PA1 and PA1PolβΔ cells were treated with a 5 Gy dose. (C) PA1 and PA1PolβΔ cells were treated with a 10 Gy dose. (D) PA1 and PA1PolβΔ cells were treated with a 15 Gy of dose.

Article Snippet: PA1 cells were obtained from the National Centre for Cell Science, Pune, India.

Techniques: Flow Cytometry, Standard Deviation, Control

Journal: American Journal of Cancer Research

Article Title: Different mechanisms underlie IL-6 release in chemosensitive and chemoresistant ovarian carcinoma cells

doi:

Figure Lengend Snippet: IL-6 intracellular levels in PEA1 and PEA2 cells. Cells (1×105/mL) were incubated in RPMI 1640 medium at 37°C in a 5% CO2 atmosphere for 6 hrs. (A) Supernatants were collected and analysed for IL-6 content by ELISA. (B) Effect of AKT or JNK inhibitors on IL-6 release by PEA1 and PEA2 cells. Cells (1×105/mL) were incubated in RPMI 1640 medium, in the presence or absence of 4 μM AKT inhibitor (AKT Inhibitor IV, Calbiochem) or 10 μM JNK inhibitor (SP600125, Sigma-Aldrich) at 37°C in a 5% CO2 atmosphere for 6 hrs. Cell lysates were analysed by western blot with an anti-IL-6 (ab6672, Abcam), an anti-pAKT (Ser473) (#9271, Cell signaling), an anti-AKT (#9272, Cell signaling) or an anti-GAPDH (sc-32233, Santa Cruz Biotechnology) antibody. (C) Supernatants from (B) were collected and IL-6 content was analysed by ELISA. All data were obtained from triplicate samples and confirmed in three separate experiments.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: American Journal of Cancer Research

Article Title: Different mechanisms underlie IL-6 release in chemosensitive and chemoresistant ovarian carcinoma cells

doi:

Figure Lengend Snippet: AKT affect IL-6 secretion in PEA1 cells. Cells (1×105/mL) were incubated in RPMI 1640 medium, in the presence or absence of AKT inhibitors at different concentrations for 6 hrs. (A) Cell lysates were analysed by western blot as described in Figure 2. (B) Supernatants were collected and IL-6 content was analysed by ELISA. Data are from triplicate samples and confirmed in two separate experiments. (C) PEA1 and PEA2 cells were transfected with akt siRNAs or a non-targeted (NT) siRNA at a final concentration of 50 nmol/L for 48 hours. Next, the cells were harvested, lysed and analysed by Western blotting with the indicated antibodies. (D) Supernatants from (C) were collected and IL-6 content was analysed by ELISA. Data were obtained from triplicate samples and confirmed in two separate experiments.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay, Transfection, Concentration Assay